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It has been experimentally shown that Ca uptake and mitochondrial metabolism underlie Treg survival in OS conditions (131)

This reduces the susceptibility of the immortalized cells to drug toxicity and even switches the mode of cell death from necrosis in primary hepatocytes to caspase-dependent apoptosis in hepatoma cells [140, 141]

In addition, demographic diversity is not mentioned in most of the studies, which may affect the applicability of the findings to various populations

However, the inclusion of the control group in the experimental protocol and examination of a big number of rams may be important to be conducted in a further study