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The resulting solution was purified using EMD Millipore Amicon Ultra-0.5 centrifugal filter units with a membrane molecular weight cut-off (MWCO) of 10 kDa were used to remove residual ions (i.e

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One PLM neuron of the adult Day 1 animal was laser-ablated, and the animals recovered on OP50 seeded NGM agar plates for 36 h before testing

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