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On day 20 of the differentiation procedure, the cells were washed 4 times with PBS and then treated with L15 medium with dihexa (without Hydrocortisone 21-hemisuccinate or dexamethasone) supplemented with 25 M Rifampicin (Sigma)
both are WADA-banned
Proliferating cellular nuclear antigen (PCNA) inhibits G1 and G2 cell cycle phases and DNA replication
however, in September 2024, after further investigation, ipamorelin was removed from this list
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