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For sequential image acquisition, the following imaging parameters were used: excitation 405 nm (emission 450/50 nm) for Hoechst and excitation 561 nm (emission 600/50 nm) for Alexa Fluor 568 with 50 ms and 200 ms exposure time, respectively

Emerging GLP-1 and glucagon receptor co-agonists Multiple GLP-1R co-agonists are emerging for the treatment of obesity and diabetes